4YRY

Insights into flavin-based electron bifurcation via the NADH-dependent reduced ferredoxin-NADP oxidoreductase structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-09
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43
Unit cell lengths	81.400, 81.400, 307.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.400
R-factor	0.2039
Rwork	0.200
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ylf
RMSD bond length	0.005
RMSD bond angle	0.909
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	76947
<I/σ(I)>	14.2	2.3
Completeness [%]	99.1	97.1
Redundancy	5.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.225M NH4H2PO4, 5 % (v,v) Ethanol, 20 % glycerol. soaked for 40 min in 5 mM NADH