4YRH

p21 isoform of MEC-17 from Danio Rerio

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	74.374, 66.299, 75.841
Unit cell angles	90.00, 103.84, 90.00

Refinement procedure

Resolution	49.272 - 2.861
R-factor	0.2214
Rwork	0.218
R-free	0.25570
RMSD bond length	0.005
RMSD bond angle	0.925
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	32007
<I/σ(I)>	18.57
Completeness [%]	98.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	25% PEG3350, 0.1M HEPES, 0.2M (NH4)2SO4