4YPN
Crystal structure of a LonA fragment containing the 3-helix bundle and the AAA-alpha/beta domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2013-01-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 59.203, 63.892, 69.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.070 |
| R-factor | 0.18747 |
| Rwork | 0.185 |
| R-free | 0.23657 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m6a |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.892 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.086 | 0.575 |
| Number of reflections | 15628 | |
| <I/σ(I)> | 19.49 | 4.62 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 295 | 0.1 M sodium citrate pH 5.5, 25 % PEG 3350 |
