4YPD
Crystal Structure of DAPK1 catalytic domain in complex with the hinge binding fragment 4-methylpyridazine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.853, 62.511, 88.193 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.460 - 1.400 |
R-factor | 0.1562 |
Rwork | 0.155 |
R-free | 0.17720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jks |
RMSD bond length | 0.010 |
RMSD bond angle | 1.443 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 57841 | |
<I/σ(I)> | 23.33 | |
Completeness [%] | 99.5 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES, 1.7 M ammonium sulfate |