4YP7
Crystal structure of Methanobacterium thermoautotrophicum NMNAT in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 124.448, 124.448, 111.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.1909 |
| Rwork | 0.190 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ej2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.566 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.990 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.217 |
| Number of reflections | 41867 | |
| <I/σ(I)> | 18.8 | 6.09 |
| Completeness [%] | 93.1 | 93.3 |
| Redundancy | 1.56 | 1.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | ammonium sulfate, glycerol, TRIS |
