4YOY

Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 in complex with poly-dT and Mg2+ ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	94.878, 94.891, 106.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.911 - 1.950
R-factor	0.1814
Rwork	0.180
R-free	0.20420
RMSD bond length	0.008
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.836	19.911	2.060
High resolution limit [Å]	1.950	6.170	1.950
Rmerge		0.039	0.843
Rmeas	0.095
Rpim	0.036	0.017	0.360
Total number of observations	494382	14162	64743
Number of reflections	70614
<I/σ(I)>	13	35.7	2.4
Completeness [%]	99.9	96.8	100
Redundancy	7	6	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	MES, PEG6000, LiCl