4YOV

Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 in complex with poly-dA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	94.676, 95.099, 105.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 2.050
R-factor	0.1931
Rwork	0.191
R-free	0.23250
RMSD bond length	0.008
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.567	19.800	2.160
High resolution limit [Å]	2.050	6.480	2.050
Rmerge		0.029	0.485
Rmeas	0.062
Rpim	0.024	0.012	0.259
Total number of observations	403350	13238	36288
Number of reflections	59879
<I/σ(I)>	17.1	48	2.8
Completeness [%]	99.3	96.2	96.4
Redundancy	6.7	6.6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	MES, PEG6000, LiCl