4YOS

p107 pocket domain complexed with LIN52 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	CCD
Collection date	2013-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	75.430, 101.060, 140.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.449 - 2.300
R-factor	0.2033
Rwork	0.199
R-free	0.24240
RMSD bond length	0.005
RMSD bond angle	0.920
Data reduction software	iMOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	60.500
High resolution limit [Å]	2.300
Number of reflections	24232
<I/σ(I)>	7.1
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	MES, PEG 400 4%, 1.6M ammonium sulfate