4YOO

p107 pocket domain in complex with LIN52 P29A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.984
Spacegroup name	C 2 2 21
Unit cell lengths	74.815, 100.530, 142.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.395 - 2.400
R-factor	0.2002
Rwork	0.195
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.091
Data reduction software	iMOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	71.400
High resolution limit [Å]	2.400
Number of reflections	21369
<I/σ(I)>	6.6
Completeness [%]	99.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	MES, PEG 400 4%, 1.6M ammonium sulfate