4YNZ

Structure of the N-terminal domain of SAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-16
Detector	RAYONIX MX-225
Wavelength(s)	0.97916
Spacegroup name	P 1
Unit cell lengths	43.299, 60.745, 73.937
Unit cell angles	103.74, 106.48, 107.42

Refinement procedure

Resolution	29.675 - 2.000
R-factor	0.192
Rwork	0.190
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hak
RMSD bond length	0.008
RMSD bond angle	1.110
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.087	0.460
Number of reflections	42329
<I/σ(I)>	8.2	1.3
Completeness [%]	96.2	85.7
Redundancy	2.3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.1 M Tris, 15% PEG MME 2000