4YNZ
Structure of the N-terminal domain of SAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-16 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.299, 60.745, 73.937 |
| Unit cell angles | 103.74, 106.48, 107.42 |
Refinement procedure
| Resolution | 29.675 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hak |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.460 |
| Number of reflections | 42329 | |
| <I/σ(I)> | 8.2 | 1.3 |
| Completeness [%] | 96.2 | 85.7 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris, 15% PEG MME 2000 |
