4YNX
Structure of YdiE from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2003-03-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.736, 38.189, 54.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.500 - 1.500 |
| R-factor | 0.175 |
| Rwork | 0.171 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MOLECULAR DYNAMICS SIMULATION OF PDB 2JRA |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.028 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 1.554 |
| High resolution limit [Å] | 1.497 | 1.497 |
| Rmerge | 0.063 | 0.346 |
| Number of reflections | 12710 | |
| <I/σ(I)> | 19.3 | 4.1 |
| Completeness [%] | 98.4 | 95.8 |
| Redundancy | 4.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.6 M ammonium sulphate, 0.1M Tris |
