4YNW
Assembly Chaperone of RpL4 (Acl4) (Residues 1-338)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.134, 49.566, 80.314 |
| Unit cell angles | 98.59, 100.11, 98.53 |
Refinement procedure
| Resolution | 19.616 - 2.950 |
| R-factor | 0.2382 |
| Rwork | 0.235 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ynv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.660 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 16367 | |
| <I/σ(I)> | 40.3 | 2 |
| Completeness [%] | 97.2 | 85.8 |
| Redundancy | 7.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20 % (w/v) PEG 3350, 0.2 M ammonium citrate tribasic |
