4YNH

Structure of the C. elegans SAS-5 Implico dimerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	27.180, 36.290, 42.600
Unit cell angles	90.00, 97.50, 90.00

Refinement procedure

Resolution	18.486 - 1.000
R-factor	0.1291
Rwork	0.128
R-free	0.14910
RMSD bond length	0.007
RMSD bond angle	1.152
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.235	18.486	1.050
High resolution limit [Å]	1.000	3.160	1.000
Rmerge		0.094	0.763
Rmeas	0.116
Rpim	0.044	0.038	0.314
Total number of observations	305514	10632	43323
Number of reflections	44263
<I/σ(I)>	12	26.5	4.2
Completeness [%]	99.4	99.6	98.3
Redundancy	6.9	7.2	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M MES/imidazole pH 6.4, 10% w/v PEG 20,000, 20% v/v PEG MME 550, 0.1 M carboxylic acids mixture