4YNA
Oxidized YfiR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 119.668, 119.668, 85.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.880 - 3.200 |
| R-factor | 0.2479 |
| Rwork | 0.246 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yn7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
| Rmerge | 0.164 | 0.054 | 0.486 |
| Total number of observations | 74115 | ||
| Number of reflections | 10704 | ||
| <I/σ(I)> | 5.1 | ||
| Completeness [%] | 98.9 | 94.4 | 100 |
| Redundancy | 6.9 | 6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 0.1 M HEPES pH 7.5, 1.8 M Ammonium sulfate |
