4YN7
Non-oxidized YfiR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97889 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 120.725, 120.725, 87.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.679 - 2.600 |
| R-factor | 0.1916 |
| Rwork | 0.189 |
| R-free | 0.23380 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.088 | 0.042 | 0.497 |
| Total number of observations | 581053 | ||
| Number of reflections | 20544 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 99.9 | 97.9 | 100 |
| Redundancy | 28.3 | 23.4 | 28.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 0.1 M HEPES pH 7.5, 1.8 M Ammonium sulfate |
