4YN6

Structural Insight reveals dynamics in repeating r(CAG) transcript found in Huntington's disease (HD) and Spinocerebellar ataxias (SCAs)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-05
Detector	ADSC QUANTUM 1
Wavelength(s)	0.99
Spacegroup name	H 3
Unit cell lengths	46.074, 46.074, 133.202
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.401 - 2.301
R-factor	0.2189
Rwork	0.217
R-free	0.26070
RMSD bond length	0.005
RMSD bond angle	1.387
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	Coot
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.070
Number of reflections	7642
<I/σ(I)>	21.3	7
Completeness [%]	99.4	99.4
Redundancy	3.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50mM Tris HCl, 25mM Magnesium Sulphate and 1.8M Ammonium Sulfate