4YMW
Crystal structure of an amino acid ABC transporter with histidines
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.247, 115.264, 301.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.722 - 2.804 |
| R-factor | 0.2269 |
| Rwork | 0.224 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dhw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.490 | |
| Number of reflections | 38389 | |
| <I/σ(I)> | 22 | 2.1 |
| Completeness [%] | 92.9 | 74.4 |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% PEG4000, 0.1M NaCl, 0.1M MgCl, 0.1M Na Citrate |
