4YMR
Crystal structure of the domain swapped PXB/TPR domain of mouse SNX21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 100.920, 100.920, 63.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.487 - 2.400 |
R-factor | 0.2298 |
Rwork | 0.228 |
R-free | 0.26720 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.368 |
Data scaling software | Aimless |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 21.500 |
High resolution limit [Å] | 2.400 |
Number of reflections | 13890 |
<I/σ(I)> | 10.9 |
Completeness [%] | 93.0 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% w/v PEG 8000, 20% v/v ethylene glycol, 0.1 M MES/imidazole (pH 6.5) 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium l-tartrate, 0.02 M sodium oxamate |