4YMG
Crystal structure of SAM-bound Podospora anserina methyltransferase PaMTH1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.040, 78.791, 82.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.359 - 1.899 |
R-factor | 0.1605 |
Rwork | 0.159 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qvk |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | XDS (xdsapp 1.1) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.520 | 1.946 |
High resolution limit [Å] | 1.899 | 1.899 |
Rmerge | 0.137 | |
Number of reflections | 39322 | |
<I/σ(I)> | 12.75 | 2 |
Completeness [%] | 99.8 | 98 |
Redundancy | 6.63 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.2 M Lithium sulfate, 0.1M Bis-Tris, 25% PEG3350 |