4YMD
CL-K1 trimer bound to man(alpha1-2)man
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.850, 106.650, 78.390 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 44.080 - 2.870 |
| R-factor | 0.2157 |
| Rwork | 0.213 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yli |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.853 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.080 | 2.973 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.052 | 0.537 |
| Number of reflections | 24670 | |
| <I/σ(I)> | 11.2 | 1.8 |
| Completeness [%] | 91.0 | 93.57 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES, 10% v/v ethanol and 4 mM calcium chloride |
