4YLY

Crystal structure of peptidyl-tRNA hydrolase from a Gram-positive bacterium, staphylococcus aureus at 2.25 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	46.752, 70.604, 128.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.070 - 2.250
R-factor	0.19575
Rwork	0.193
R-free	0.24313
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ryn
RMSD bond length	0.010
RMSD bond angle	1.393
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.250
Number of reflections	21922
<I/σ(I)>	15.27
Completeness [%]	99.6
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	25% PEG 3350, 0.2 M ammonium sulfate, 0.1 M Hepes PH 7.5