4YLS

Tubulin Glutamylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	C 2 2 21
Unit cell lengths	79.126, 122.324, 129.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.360 - 2.600
R-factor	0.212
Rwork	0.210
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.591
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.144	0.091	0.528
Rmeas	0.152	0.097	0.555
Rpim	0.046	0.032	0.162
Total number of observations	193516
Number of reflections	19228
<I/σ(I)>	11.5
Completeness [%]	97.8	99.6	84.6
Redundancy	10.1	9.4	9.7
CC(1/2)		0.983	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		279	PEG 8000, magnesium acetate