4YLN
E. coli Transcription Initiation Complex - 17-bp spacer and 4-nt RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 237.401, 206.052, 248.690 |
| Unit cell angles | 90.00, 116.55, 90.00 |
Refinement procedure
| Resolution | 39.900 - 5.500 |
| R-factor | 0.24889 |
| Rwork | 0.245 |
| R-free | 0.32811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.488 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 5.590 |
| High resolution limit [Å] | 5.500 | 5.500 |
| Number of reflections | 69849 | |
| <I/σ(I)> | 9.4 | 0.71 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 7% PEG3350, 150 mM magnesium chloride, 100 mM HEPES sodium, pH 7.0 |
