4YLI
CL-K1 trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.990, 107.590, 77.820 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 48.208 - 2.450 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b08 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.616 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 2.538 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.054 | 0.476 |
| Number of reflections | 42224 | |
| <I/σ(I)> | 10.09 | 2.03 |
| Completeness [%] | 95.5 | 87.15 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES pH 7.0 10% v/v ethanol and 4 mM calcium chloride |
