4YLF

Insights into flavin-based electron bifurcation via the NADH-dependent reduced ferredoxin-NADP oxidoreductase structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-08
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43
Unit cell lengths	81.420, 81.420, 311.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.294 - 2.301
R-factor	0.191
Rwork	0.187
R-free	0.21890
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	86542
<I/σ(I)>	8.4	1.4
Completeness [%]	96.9	86.2
Redundancy	2.9	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M NH4H2PO4