4YLC

Crystal Structure of Del-C4 mutant of hsp14.1 from Sulfolobus solfatataricus P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97913
Spacegroup name	I 4
Unit cell lengths	189.105, 189.105, 88.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.572 - 3.100
R-factor	0.2413
Rwork	0.240
R-free	0.27150
RMSD bond length	0.004
RMSD bond angle	0.854
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.340
High resolution limit [Å]	3.100	3.100
Rmerge	0.038	0.330
Number of reflections	28555
<I/σ(I)>	19.6
Completeness [%]	99.7	98.9
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	12% w/v polyethylene glycol 3350, 0.1M sodium malonate (pH 7.0)