4YKL
Hnt3 in complex with DNA and guanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 119.083, 119.083, 36.564 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.540 - 2.250 |
| R-factor | 0.1836 |
| Rwork | 0.180 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xba |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.540 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.073 | 0.722 |
| Number of reflections | 14369 | |
| <I/σ(I)> | 20.7 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 25% (w/v) PEG-3350, 0.15 M Mg-Acetate |
