4YKH
Thirty minutes iron loaded human H ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.050, 184.050, 184.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.070 - 1.520 |
| R-factor | 0.16583 |
| Rwork | 0.165 |
| R-free | 0.18713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ajo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.007 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 1.600 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.077 | 0.410 |
| Number of reflections | 41531 | |
| <I/σ(I)> | 25.8 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.5 | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 2.0 M magnesium chloride, 0.1 M Bicine pH 9.0 |
