4YKF
Crystal Structure of the Alkylhydroperoxide Reductase subunit F (AhpF) with NADH from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.090, 59.390, 121.350 |
| Unit cell angles | 90.00, 111.34, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5q |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.676 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (0.1.26) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.870 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.313 |
| Number of reflections | 22917 | |
| <I/σ(I)> | 10.3 | 3.5 |
| Completeness [%] | 92.3 | 85.1 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Na-Hepes, 2.5 % (v/v) PEG 400, 2 M ammonium sulfate, 10 mM cadmium chloride |
