4YKB
Structure of GUN4 from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.998, 114.998, 141.193 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.500 - 3.500 |
| R-factor | 0.25907 |
| Rwork | 0.256 |
| R-free | 0.31315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z3x |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.466 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.210 | 0.666 |
| Number of reflections | 13965 | |
| <I/σ(I)> | 5.2 | 1.9 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1:1 ratio of protein (35 mg/mL in 20 mM Tricine (pH 8.0), 2 mM beta-mercaptoethanol) to well solution (1.0 M ammonium citrate (pH 7.0), 0.1 M Bis Tris Propane (pH 7.0). Crystals only appeared after 44 weeks, likely the time taken for proteolysis, resulting in the N- and C-terminal truncations |
