4YK6

Crystal structure of APC-ARM in complexed with Amer1-A4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97884
Spacegroup name	P 21 21 21
Unit cell lengths	49.465, 71.152, 91.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.620 - 1.700
R-factor	0.199
Rwork	0.197
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nmw
RMSD bond length	0.012
RMSD bond angle	1.515
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.076	0.403
Number of reflections	36350
<I/σ(I)>	11.3
Completeness [%]	99.6	99.4
Redundancy	14.4	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	287	0.2M NACL, 10% PEG8000