4YK6
Crystal structure of APC-ARM in complexed with Amer1-A4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97884 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.465, 71.152, 91.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.620 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nmw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 0.403 |
| Number of reflections | 36350 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 14.4 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 287 | 0.2M NACL, 10% PEG8000 |
