4YJM

The apo structure of Agrobacterium tumefaciens ClpS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	58.007, 92.550, 96.464
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.543 - 1.952
R-factor	0.1789
Rwork	0.177
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dnj
RMSD bond length	0.012
RMSD bond angle	1.176
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall
Low resolution limit [Å]	18.543
High resolution limit [Å]	1.952
Number of reflections	38281
<I/σ(I)>	34.74
Completeness [%]	99.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	2.2M Ammonium Sulfate 0.2M Sodium Formate