4YJ0
Crystal structure of the DM domain of human DMRT1 bound to 25mer target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.23 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.185, 138.926, 141.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.731 - 3.814 |
| R-factor | 0.2323 |
| Rwork | 0.229 |
| R-free | 0.26240 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.895 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | RESOLVE |
| Refinement software | PHENIX (dev_1801) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.897 | 4.050 |
| High resolution limit [Å] | 3.814 | 3.810 |
| Rmerge | 0.108 | 0.940 |
| Number of reflections | 8170 | |
| <I/σ(I)> | 9.5 | 1.9 |
| Completeness [%] | 98.6 | 94 |
| Redundancy | 6.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M BitsTris pH 7.5, 10% MPD, 7-11% PEG 3350, 10uM zinc chloride |
