4YIQ
Structure of the CEACAM6-CEACAM8 heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.465, 97.192, 97.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.900 - 1.850 |
| R-factor | 0.1871 |
| Rwork | 0.186 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4Y88 4Y8A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.900 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 1.341 | |
| Number of reflections | 51361 | |
| <I/σ(I)> | 11.3 | 1.2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | Ratio of protein:reservoir = 1:0.2 Reservoir solution; 20% PEG 8000, 0.2 M sodium chloride, 0.1 M Sodium Phosphate/Citric Acid, pH 4.2 |
