4YHW
Yeast Prp3 (296-469) in complex with fragment of U4/U6 di-snRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97968 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.672, 59.565, 109.783 |
| Unit cell angles | 90.00, 118.52, 90.00 |
Refinement procedure
| Resolution | 29.782 - 3.250 |
| R-factor | 0.251 |
| Rwork | 0.248 |
| R-free | 0.29930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yhu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.330 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.013 | 0.106 |
| Number of reflections | 24895 | |
| <I/σ(I)> | 5.38 | 0.9 |
| Completeness [%] | 97.9 | 98.5 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium fluoride 0.18 M DL-malic acid, pH 6.8 13.2 % PEG 3350 |
