4YHG
NATIVE BACTEROIDETES-AFFILIATED GH5 CELLULASE LINKED WITH A POLYSACCHARIDE UTILIZATION LOCUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.326, 99.553, 102.462 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.400 - 2.400 |
R-factor | 0.1869 |
Rwork | 0.185 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhe |
RMSD bond length | 0.010 |
RMSD bond angle | 1.316 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.21) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 71.401 | 49.777 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Rmerge | 0.056 | 0.496 | |
Rmeas | 0.116 | ||
Rpim | 0.048 | 0.028 | 0.234 |
Total number of observations | 180101 | 5722 | 25018 |
Number of reflections | 34325 | ||
<I/σ(I)> | 10.2 | 21.1 | 3.1 |
Completeness [%] | 99.8 | 99.3 | 99.8 |
Redundancy | 5.2 | 4.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M potassium nitrate, 20 % w/v PEG 3350, 5mM Cellotetraose |