4YGI

Crystal Structure of SUVH5 SRA bound to fully hydroxymethylated CG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0718
Spacegroup name	P 42 21 2
Unit cell lengths	76.980, 76.980, 72.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.956 - 2.600
R-factor	0.2367
Rwork	0.234
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q0b
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.066	0.035	0.750
Total number of observations	32545
Number of reflections	7060
<I/σ(I)>	21.7		2.4
Completeness [%]	98.8	95.8	99.1
Redundancy	4.6	4.1	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M Sodium chloride, 0.1M BIS-TRIS pH 6.5, 25%(w/v) Polyethylene glycol 3,350