4YG7
Structure of FL autorepression promoter complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 228.200, 228.200, 130.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 161.400 - 3.770 |
| Rwork | 0.384 |
| R-free | 0.37850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dnv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.406 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 161.400 | |
| High resolution limit [Å] | 3.770 | |
| Number of reflections | 34997 | |
| <I/σ(I)> | 4 | 1.4 |
| Completeness [%] | 63.6 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | sodium formate |
