4YG3
Structural basis of glycan recognition in neonate-specific rotaviruses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.812, 64.734, 36.752 |
| Unit cell angles | 90.00, 114.87, 90.00 |
Refinement procedure
| Resolution | 29.643 - 2.285 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.701 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.112 | 0.223 |
| Number of reflections | 5508 | |
| <I/σ(I)> | 10.8 | 5.8 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris, 1.6 M Ammonium sulfate |
