4YG1

HipB-O1-O2 complex/P21212 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	293.887, 54.460, 47.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.900 - 3.250
Rwork	0.246
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dnv
RMSD bond length	0.009
RMSD bond angle	1.271
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	146.900
High resolution limit [Å]	3.250
Rmerge		0.327
Number of reflections	11197
<I/σ(I)>	8	2
Completeness [%]	95.6
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	38% MPD, 0.1 M sodium acetate, pH 4.6