4YG1
HipB-O1-O2 complex/P21212 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 293.887, 54.460, 47.665 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 146.900 - 3.250 |
Rwork | 0.246 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dnv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.271 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 146.900 | |
High resolution limit [Å] | 3.250 | |
Rmerge | 0.327 | |
Number of reflections | 11197 | |
<I/σ(I)> | 8 | 2 |
Completeness [%] | 95.6 | |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 38% MPD, 0.1 M sodium acetate, pH 4.6 |