4YFZ
Structural basis of glycan recognition in neonate-specific rotaviruses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.960, 55.280, 56.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.095 - 1.500 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.123 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.100 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.125 | 0.245 |
| Number of reflections | 28272 | |
| <I/σ(I)> | 10.6 | 6.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.94 M Sodium citrate 0.1 M Imidazole |
