4YFG
Crystal structure of PTP delta meA3/meB minus variant Ig1-Fn1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-31 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.491, 72.628, 94.407 |
| Unit cell angles | 107.65, 94.44, 108.47 |
Refinement procedure
| Resolution | 48.406 - 3.491 |
| R-factor | 0.2919 |
| Rwork | 0.291 |
| R-free | 0.31480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd6 2yd9 4yfe |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.037 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.491 | 3.500 |
| Rmerge | 0.491 | |
| Number of reflections | 18642 | |
| <I/σ(I)> | 4.8 | 1.9 |
| Completeness [%] | 91.4 | 84.2 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% polyethylene glycol (PEG) 3350, 0.1M Ammonium iodide |
