4YF1
1.85 angstrom crystal structure of lmo0812 from Listeria monocytogenes EGD-e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.811, 58.675, 95.730 |
| Unit cell angles | 90.00, 114.31, 90.00 |
Refinement procedure
| Resolution | 29.340 - 1.850 |
| R-factor | 0.18164 |
| Rwork | 0.180 |
| R-free | 0.20801 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.085 | 0.643 |
| Number of reflections | 75985 | |
| <I/σ(I)> | 31.6 | 2.36 |
| Completeness [%] | 99.2 | 92.9 |
| Redundancy | 6.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M tri-sodium citrate, pH 5.5, 20% (w/v) PEG 3000 |
