4YEI
Beta1mut synthetic solenoid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-29 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.541870 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 110.360, 115.960, 164.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.430 - 3.550 |
| R-factor | 0.21967 |
| Rwork | 0.218 |
| R-free | 0.24494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.617 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.430 | 3.650 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.160 | 1.030 |
| Number of reflections | 12716 | |
| <I/σ(I)> | 4.8321 | 2.08 |
| Completeness [%] | 98.0 | 97.98 |
| Redundancy | 4.66 | 4.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 80 mM MES pH 6.5, 128 mM magnesium sulfate. |
