4YEG
Characterisation of Polyphosphate Kinase 2 from the Intracellular Pathogen Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 273.15 |
| Detector technology | PIXEL |
| Collection date | 2013-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.790, 88.890, 163.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.638 - 2.230 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3czq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.464 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.052 | 0.539 |
| Number of reflections | 61793 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294.15 | 0.8 M Na Citrate, 0.1 M Na HEPES, 2.5 mM AMP-PNP, 1 mM MgCl2 |
