4YEE
beta2 carbohydrate binding module (CBM) of AMP-activated protein kinase (AMPK) in complex with glucosyl-beta-cyclodextrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 204.538, 96.432, 118.724 |
| Unit cell angles | 90.00, 125.46, 90.00 |
Refinement procedure
| Resolution | 43.380 - 2.000 |
| R-factor | 0.1715 |
| Rwork | 0.169 |
| R-free | 0.22463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f15 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.380 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.668 |
| Number of reflections | 127116 | |
| <I/σ(I)> | 16.9 | 2.8 |
| Completeness [%] | 99.9 | 97.8 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281.15 | in 0.2 M lithium chloride, 20% w/v PEG 6000 and 0.1 M sodium HEPES pH 7 |
