4YDX
Crystal structure of cisplatin bound to a human copper chaperone (monomer) - new refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90511 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.070, 54.070, 55.578 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.030 - 1.602 |
| R-factor | 0.1369 |
| Rwork | 0.136 |
| R-free | 0.15550 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3iwl |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.118 | 0.503 |
| Number of reflections | 13182 | |
| <I/σ(I)> | 24.9 | 3.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 19.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 2M lithium sulfate, 0.1M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
