4YDS
FlaH from Sulfolobus acidocaldarius with ATP and Mg-Ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.351, 52.945, 73.590 |
| Unit cell angles | 90.00, 130.78, 90.00 |
Refinement procedure
| Resolution | 42.933 - 2.300 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dr3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.976 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 11612 |
| <I/σ(I)> | 19.3 |
| Completeness [%] | 85.5 |
| Redundancy | 2.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100mM HEPES pH 7.0, 32.5% PEG 3350, 150mM NaSCN |
