4YDR
Crystal structure of oxidized homoserine dehydrogenase of Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.818, 78.909, 65.814 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 28.500 - 1.600 |
| R-factor | 0.19811 |
| Rwork | 0.197 |
| R-free | 0.22799 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.078 | 0.295 |
| Number of reflections | 74576 | |
| <I/σ(I)> | 38.8 | 7.2 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 4.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG 3350, magnesium chloride, PEG 400, DMSO |
