4YDP
Crystal structure of N-terminal PDZ domain of ZASP in complex with myotilin C-terminal peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.900, 95.500, 28.900 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 47.750 - 1.400 |
| R-factor | 0.1252 |
| Rwork | 0.124 |
| R-free | 0.15650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pkt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.750 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.048 | 0.301 |
| Number of reflections | 24577 | |
| <I/σ(I)> | 14.7 | 2 |
| Completeness [%] | 92.4 | 61.8 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.15 | PEG 6000, imidazole |
